Recently Xiaobian received a lot of questions, one of which is the following Xiaobian for you to sort out the steps of cellulose acetate film electrophoresis steps, I hope these methods can help you.
First of all, the filter paper strip of appropriate size is cut to build the filter paper bridge, and then the buffer is poured into the electrophoresis tank, and the dot sample is marked on the cellulose acetate film without surface.
Then, the surface is immersed in the buffer for about twenty minutes, and when it is fully saturated, the excess buffer is removed.
Then, an appropriate amount of protein sample is taken with the sampler and uniformly added to the point transect line to form a straight line with a certain width and uniform thickness.
Then, the point sample end is placed on the negative electrode, face-down, flat on the filter paper bridge, after the balance is completed, the voltage is adjusted and the electrophoresis begins.
Then, after the electrophoresis is completed, carefully remove the film, immerse it in the dyeing solution for two minutes, and then rinse it repeatedly in the bleaching solution until it is clean, and then observe the results of the electrophoresis.
Finally, it should be noted that the acetate fiber film has poor water absorption, and the water in the process of electrophoresis is easy to evaporate and the electrophoresis is terminated, so the electrophoresis process should be carried out in a closed electrophoresis tank to ensure that it is in a humid state.
The above methods are collected by small series, and I hope to help you.